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KMID : 0368420060490010088
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Journal of Plant Biology
2006 Volume.49 No. 1 p.88 ~ p.96
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Sscp analysis of variations in haplotypes of citrus tristeza virus isolated from yuzu (Citrus junos) in geographically separate regions of Korea
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Kim Dae-Hyun
Shim Hye-Kyung
Hyeon Jae-Wook
Kwon Hyeog-Mo
Kim Kwang-Sik
Choi Min-Su
Lee Jin-Kyung
Kim Dong-Giun
Yang Ju-Sung
Lee Suk-Chan
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Abstract
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Yuzu (Cittus junos) trees were examined from six geographically separate provinces in the Republic of Korea, including four islands (Geoje, Namhae, Wan, and Jeju), 1 peninsula (Goheung), and 1 inland area (Boseong). The population of sequence variants of citrus tristeza virus (CTV) was isolated and analyzed by single-strand conformation polymorphism (SSCP) analysis of cDNA from thep20 gene. SSCP profiles of 65 PCR products showed different band patterns but with similar intensities. Sixteen haplotypes were subgrouped according to their SSCP profiles and severity of symptoms. Their genomes were sequenced and compared. DNA analysis of thep20 genes revealed nucleotide identities ranging from 88-99.8%. Based on SSCP analysis, the pathologically mild isolates of CTV yielded two to three DNA bands, whereas the most virulent isolates contained more than two bands. Comparisons of these physically separate haplotypes suggest that CTV isolates with multiple SSCP profiles could have arisen as a result of a mixed infection and genetic recombination of two divergent isolates. Plants with severe disease symptoms, such as stem pitting, closely corresponded to a CTV strain showing typical SSCP profiles in Florida (USA) and Japan.
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KEYWORD
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citrus tristeza virus, p20 gene, single-strand conformation polymorphism analysis, viral RNA population
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